FastQCFastQC Report
Mon 6 Jun 2016
ERR1379399.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379399.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244947
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTATGAAATCTCGTATGCC12510.10048620543685796Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1700.042.3521541
CGTATGC1800.040.00085444
CGTATGA1900.039.07821332
CTCGTAT1900.037.89554642
CCGTATG1900.037.89402431
TCGTATG1950.036.9238743
GACCGTA1950.036.92238629
CCGAGAC1950.036.92238625
AGACCGT2050.035.12129228
ATCTCGT2150.034.53423740
GCGGTTC2100.034.2850728
ACCGTAT2150.033.48774730
CGGTTCA2200.032.7266589
AGAGCGG2350.030.6414155
AATCTCG2450.030.30555539
GAGCGGT2400.030.0018466
AGCGGTT2450.029.3872057
AATGCCG2500.028.79945821
GAGACCG2600.027.6917927
CGGAAGA2650.027.1725751
Produced by FastQC (version 0.11.5)