FastQCFastQC Report
Mon 6 Jun 2016
ERR1379396.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379396.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808888
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATTAAGATCTCGTATGCC10630.13141497957690063Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATTA1300.038.07655731
GACCGAT1350.036.66631329
CGATTAA1350.036.66631332
CTCGTAT1300.036.34580242
CGTATGC1350.034.9996644
AGACCGA1450.034.13760428
GAGACCG1550.033.38677627
ACCGATT1500.032.99968330
TCTCGTA1450.032.58589641
TCGTATG1500.031.49969943
GCGGTTC1750.028.2854428
AGCGGTT1750.028.2854427
CCGAGAC1750.028.28544225
AAGAGCG1800.027.5014364
AGAGCGG1800.027.5014365
ATCTCGT1750.026.9997440
GAGCGGT1850.026.75656
CGGAAGA1900.026.0556031
CGGTTCA1900.026.0523829
AATGCCG1950.025.3843721
Produced by FastQC (version 0.11.5)