FastQCFastQC Report
Mon 6 Jun 2016
ERR1379395.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379395.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800094
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAATAACATCTCGTATGCC15230.19035263356555604Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA2050.038.41356741
TCGTATG2100.036.4275643
CTCGTAT2150.035.58040642
ATCTCGT2250.034.99902740
GCGGTTC2350.033.509718
AGACCGA2300.033.25994528
AGAGCGG2400.032.8177385
GAGCGGT2400.032.813646
ACCGAAT2350.032.55228830
CGAATAA2350.032.55228832
AGCGGTT2450.032.1419647
CGTATGC2400.031.87411544
CATCTCG2400.031.87411539
CCGAGAC2400.031.87411525
GACCGAA2450.031.22362129
CCGAATA2450.031.22362131
GAGACCG2550.029.99916627
CGGTTCA2700.029.9991659
AAGAGCG2650.029.721734
AATGCCG2650.029.71615421
Produced by FastQC (version 0.11.5)