FastQCFastQC Report
Mon 6 Jun 2016
ERR1379390.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379390.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1235494
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1700.037.0579944
ACGGTAC250.002107402535.99918712
CTCGTAT1850.035.26947442
TCTCGTA1800.034.99921441
GCGGTTC1850.034.0532888
ACCGGCA1900.033.15714630
GACCGGC1950.032.30696529
TCGTATG1950.032.30696543
CGGCAGT2000.031.4992932
AGCGGTT2050.030.7310167
AGACCGG2050.030.73101628
GAGCGGT2100.030.0041836
GAGACCG2100.029.99932527
CCGGCAG2150.029.30166631
AATCTCG2200.028.6357239
AGAGCGG2250.028.0039045
ATCTCGT2250.027.99936940
ATGCCGA2250.027.99936922
TGCCGAG2350.027.76533323
CGGTTCA2350.026.8079079
Produced by FastQC (version 0.11.5)