FastQCFastQC Report
Mon 6 Jun 2016
ERR1379388.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379388.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences632781
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTGTGAATCTCGTATGCC10770.1702010648233749Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTGT1350.036.6665431
GACCGGT1300.036.34602429
CTCGTAT1400.033.7498842
CGGTGTG1500.032.99988632
ACCGGTG1450.032.58609430
CGTATGC1450.032.58609444
TCGTATG1450.032.58609443
TCTCGTA1500.031.4998941
AGACCGG1650.028.63626328
ATCTCGT1750.028.28561440
GCGGTTC1750.028.2856148
GATCGGA507.15357E-526.99990718
GAGCGGT1850.026.7566626
CGGAAGA1900.026.05461
GTATGCC1900.026.05254245
AGCGGTT1950.025.3845257
CGGTTCA2050.025.2438149
GAGACCG2000.024.74991427
AGATCGG751.6956892E-623.99991617
CCGAGAC2000.023.62491825
Produced by FastQC (version 0.11.5)