FastQCFastQC Report
Mon 6 Jun 2016
ERR1379387.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379387.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477599
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGTGAATCTCGTATGCC16640.11261512764965326Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2150.038.72060844
GACCGCA2250.036.99969529
CTCGTAT2300.036.1953542
TCGTATG2300.036.1953543
TCTCGTA2400.035.62470641
ATCTCGT2500.033.2997340
GAGCGGT2600.032.8854566
AGCGGTT2650.032.263887
AGACCGC2650.031.41483328
GCGGTTC2750.031.0906528
CCGCAGT2700.030.83307831
GTATGCC2850.030.78921945
CGGAAGA2750.029.4562951
GAGACCG2850.029.21028527
CGGTTCA2950.028.9828119
ATGCCGA2950.028.98281122
AAGAGCG3000.028.5016944
AGAGCGG3000.028.5016945
AATGCCG3150.027.14263321
ACCGCAG3200.026.7185330
Produced by FastQC (version 0.11.5)