FastQCFastQC Report
Mon 6 Jun 2016
ERR1379384.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379384.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822996
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCGTAGAATCTCGTATGCC12530.1522486136020102Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1150.041.08601444
CCGCGTA1150.041.08601431
CGCGTAG1150.041.08601432
AGACCGC1250.037.79913328
ACCGCGT1300.036.3453230
TCGTATG1300.036.3453243
CTCGTAT1350.034.99919542
GCGTAGA1400.033.74922633
GACCGCG1500.031.49927529
ATCTCGT1550.030.48317140
GAGACCG1550.030.48317127
CGTAGAA1550.030.48317134
TCTCGTA1600.029.53057141
GAGCGGT1650.028.6391876
AGCGGTT1700.027.793487
AAGAGCG1750.027.0043014
AGAGCGG1750.027.0043015
CTCGTCA507.157089E-526.9993826
CCGAGAC1750.026.99937825
GCGGTTC1800.026.24948
Produced by FastQC (version 0.11.5)