FastQCFastQC Report
Mon 6 Jun 2016
ERR1379382.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379382.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367633
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAG1650.034.0906332
TCTCGTA1700.029.1174141
CGTATGC1850.027.97274644
ACCGGCT2100.027.85691830
CTCGTAT1700.027.79389242
GAGCGGT2350.026.8082946
GAGACCG2100.026.785527
TCGTATG1800.026.24978643
AGACCGG2200.025.56797428
GCGGTTC2300.025.4345748
AGAGCGG2500.025.2016375
AATGCCG2250.024.99979821
GACCGGC2350.024.89341529
ATCTCGT2100.024.64265840
CGGTTCA2450.023.8773579
CGGAAGA2600.022.5014631
AAGAGCG2700.022.5014614
AGCGGTT2700.021.6664897
ATGCCGA2600.021.6344422
CCGAGAC2700.020.83316225
Produced by FastQC (version 0.11.5)