FastQCFastQC Report
Mon 6 Jun 2016
ERR1379381.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379381.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1721436
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1750.029.57236542
CGTATGC1750.029.57150544
GACCGTC1900.028.420329
ACCGTCG1950.027.69157430
TCTCGTA2050.025.24396741
GAGCGGT2250.025.0015166
TCGTATG2150.024.06982843
CCGTCGA2250.023.99936531
CGTCGAG2350.023.93553732
GTCGAGA2200.023.52210433
ATCTCGT2250.023.0000640
AGACCGT2250.022.99939228
GCGGTTC2650.022.0748888
CGAGAAT2500.020.69945335
TCGAGAA2550.020.29358334
CGGTTCA2800.020.0887559
AGCGGTT2950.019.8299857
CGGAAGA2850.019.7409081
CCGAGAC2950.018.304625
ATGCCGA3100.018.14468222
Produced by FastQC (version 0.11.5)