FastQCFastQC Report
Mon 6 Jun 2016
ERR1379380.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379380.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685695
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACGAGTATCTCGTATGCC18440.26892423016063993Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2250.040.99960342
CCGACGA2400.038.43712631
GAGACCG2550.037.94080727
CGTATGC2450.037.65269544
GACCGAC2450.037.65269529
ACCGACG2450.037.65269530
GACGAGT2450.037.65269533
TCGTATG2500.036.89964343
ACGAGTA2500.036.89964334
CGAGTAT2550.036.17611735
CGACGAG2550.036.17611732
AGACCGA2550.036.17611728
CGGTTCA2700.034.9996579
TATCTCG2650.034.8109839
TGCCGAG2750.034.363323
CGGAAGA2700.034.1688271
TCTCGTA2700.034.16633241
AGCGGTT2800.033.7496727
GAGCGGT2900.033.3641786
AGAGCGG2850.033.159995
Produced by FastQC (version 0.11.5)