FastQCFastQC Report
Mon 6 Jun 2016
ERR1379379.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379379.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768172
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCAGAATCTCGTATGCC16560.21557671979712875Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1350.041.66630641
CCGTGCA1500.038.9996631
ATCTCGT1450.038.79276740
CTCGTAT1450.038.79276742
CGTATGC1500.037.49967244
CCGAGAC1650.035.4542425
TCGTATG1750.032.1425843
CGTGCAG1850.031.6213532
AGACCGT1850.031.6213528
ATGCCGA1950.031.15357622
AATGCCG1900.030.78920621
GAGACCG1900.030.78920627
GACCGTG1950.029.9997429
TGCCGAG1950.029.9997423
GTATGCC2000.029.24974645
GCGGTTC1950.028.8459038
GAGCGGT2050.028.5381956
AATCTCG2100.027.85690139
ACCGTGC2100.027.85690130
AGAGCGG2250.026.0014675
Produced by FastQC (version 0.11.5)