FastQCFastQC Report
Mon 6 Jun 2016
ERR1379377.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379377.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences703602
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTCTCTATCTCGTATGCC20870.2966165531081492Illumina Paired End PCR Primer 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2000.040.49955442
CGTATGC2050.039.51175744
TCTCGTA2050.039.51175741
GACCGCT2150.038.720529
GAGACCG2150.038.720527
AGACCGC2200.037.84049228
ACCGCTC2250.036.99958830
CCGCTCT2300.036.1952531
CGCTCTC2300.036.1952532
ATCTCGT2300.035.21700340
TATCTCG2300.035.21700339
CCGAGAC2400.034.6871225
TCGTATG2350.034.46770543
GTATGCC2300.034.23875445
GCGGTTC2550.033.529048
AGCGGTT2550.032.6466987
GAGCGGT2600.032.018886
CGGTTCA2650.031.4147459
CGGAAGA2600.031.1601451
AGAGCGG2850.030.0017995
Produced by FastQC (version 0.11.5)