FastQCFastQC Report
Mon 6 Jun 2016
ERR1379375.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379375.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913707
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTAGCAATCTCGTATGCC15400.16854418320096048Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA2000.042.74958430
CTCGTAT1950.040.3842242
TCGTATG1950.040.3842243
GACCGGT2100.039.6424729
TCTCGTA2050.038.4142641
GAGCGGT2200.037.8426136
CCGGTAG2200.037.8405431
CGTATGC2150.037.67405344
CGGTAGC2300.037.1735532
AGACCGG2350.035.4251928
CGGTTCA2400.034.6871649
GCGGTTC2450.033.979268
AGCGGTT2450.033.979267
CGTACCA401.5603222E-533.74967220
ATCTCGT2350.033.51031540
AGAGCGG2500.033.30155
CCGAGAC2550.032.64674425
GAGACCG2750.031.09060727
CGGAAGA2650.030.5690861
AAGAGCG2800.030.5370884
Produced by FastQC (version 0.11.5)