FastQCFastQC Report
Mon 6 Jun 2016
ERR1379374.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379374.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793156
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATAGCGATCTCGTATGCC18340.23122815688212658Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAT1750.042.42631529
ACCGATA1800.041.24780330
CCGATAG1850.040.13331
TAGCGAT1850.040.13335
TCTCGTA1900.037.89510741
ATAGCGA2000.037.12302434
TCGTATG2000.037.12302443
CTCGTAT1950.036.9234442
CGTATGC2050.036.21758344
AGACCGA2050.036.21758328
CGATAGC2050.036.21758332
GAGACCG2250.033.9981927
GCGGTTC2200.033.7482038
GATAGCG2200.033.74820333
AGAGCGG2250.033.0086485
AGCGGTT2250.032.9982457
CGGTTCA2400.031.8733029
CGGAAGA2350.031.6100031
AAGAGCG2350.031.6040234
GAGCGGT2350.031.5980456
Produced by FastQC (version 0.11.5)