FastQCFastQC Report
Mon 6 Jun 2016
ERR1379372.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379372.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844225
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGACGATCTCGTATGCC11160.13219224732743048Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAC1250.039.59791632
TCTCGTA1150.039.1306941
CCGTAGA1300.038.07491731
GTAGACG1300.036.3442433
CTCGTAT1250.036.00023742
CGTATGC1250.035.99810444
GACCGTA1300.034.61356429
AGACGAT1300.034.61356435
TCGTATG1350.033.3315843
GACGATC1400.032.1430736
TAGACGA1550.029.0307334
ACCGTAG1550.029.0307330
GCGGTTC1600.028.1235228
GAGACCG1600.028.12352227
AGACCGT1600.028.12352228
GTATGCC1700.027.79265445
ATCTCGT1700.026.47076240
AGAGCGG1750.025.7220735
GAGCGGT1750.025.7175036
CGGAAGA1900.024.8759521
Produced by FastQC (version 0.11.5)