FastQCFastQC Report
Mon 6 Jun 2016
ERR1379371.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379371.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732131
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGACAATCTCGTATGCC10210.13945591704216867Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1600.039.37428341
CTCGTAT1750.037.28503442
CGTATGC1700.037.05814744
GACCGCA1850.034.05343229
CGCAGAC1850.034.05343232
GAGACCG2000.031.49942827
CCGCAGA2000.031.49942831
TCGTATG2100.031.07086443
AGACCGC2150.030.34828628
ATCTCGT2150.029.30179440
GACAATC2250.028.99947236
GCGGTTC2200.028.6358418
ACCGCAG2200.028.63584130
AATCTCG2400.028.12448739
CGGTTCA2250.027.9994899
AGAGCGG2300.027.3945455
GTATGCC2300.027.39080445
AAGAGCG2400.026.2531054
CCGAGAC2350.025.85059525
GAGCGGT2600.024.2336396
Produced by FastQC (version 0.11.5)