FastQCFastQC Report
Mon 6 Jun 2016
ERR1379368.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379368.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences707110
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAGTAGATCTCGTATGCC12640.17875578057162253Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1350.041.66614544
TCTCGTA1350.041.66614541
CTCGTAT1400.040.17806642
GACCGCA1400.040.17806629
CGCAGTA1500.037.4995332
TCGTATG1500.037.4995343
AGACCGC1550.036.28986728
GTATGCC1700.034.4113345
GAGCGGT1650.034.0928886
ATCTCGT1800.033.74957740
GCGGTTC1600.033.7495778
GAGACCG1600.033.74957727
ACCGCAG1700.033.0878230
CCGCAGT1750.032.14245231
GTAGATC1800.031.24960936
CGGTTCA1750.030.8567549
AGAGCGG1800.030.0017455
AGCGGTT1800.029.9996247
AAGAGCG1900.029.6069854
CGGAAGA2000.029.2558381
Produced by FastQC (version 0.11.5)