FastQCFastQC Report
Mon 6 Jun 2016
ERR1379366.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379366.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580286
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCATAGATCTCGTATGCC15550.2679713106985176Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1800.039.99869541
CGTATGC1750.039.85584344
CGGCATA1800.038.74873732
CTCGTAT1850.037.70147342
CCGGCAT1900.036.70933531
ATCTCGT1950.035.76806340
TCGTATG1950.035.76806343
GGCATAG2100.033.21320333
GCATAGA2100.033.21320334
GAGCGGT2200.032.7290276
ACCGGCA2150.032.44080430
GAGACCG2200.031.70351427
CGGTTCA2350.031.5947179
AGAGCGG2300.031.3114225
AGACCGG2250.030.99899128
GCGGTTC2300.030.3250988
CCGAGAC2300.030.32509825
GTATGCC2500.029.69903445
GACCGGC2350.029.67988629
ATAGATC2400.029.06155436
Produced by FastQC (version 0.11.5)