FastQCFastQC Report
Mon 6 Jun 2016
ERR1379363.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379363.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516277
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCATGAGATCTCGTATGCC11660.22584775227252038Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1450.040.3433541
CGTATGC1500.038.99857344
AGACCGC1600.037.96735828
TCGTATG1600.036.5611643
GACCGCA1700.035.73398629
CTCGTAT1650.035.45324742
ACCGCAT1650.035.45324730
CGCATGA1650.035.45324732
GAGACCG1650.035.45324727
ATCTCGT1700.034.41050340
GCGGTTC1750.033.4273458
CGGTTCA1800.032.498819
GTATGCC1750.032.14167845
AGCGGTT1850.031.6204647
TGCCGAG2000.031.49884623
GAGCGGT1900.030.791336
CCGAGAC1900.030.78834525
CCGCATG1900.030.78834531
AATGCCG1950.029.99890121
CGGAAGA2000.029.2602651
Produced by FastQC (version 0.11.5)