FastQCFastQC Report
Mon 6 Jun 2016
ERR1379360.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379360.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601796
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGCAGATCTCGTATGCC11140.1851125630612367Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1500.042.044
GACCGTA1600.040.7812529
CCGTAGC1650.039.54545631
TCGTATG1650.039.54545643
ATCTCGT1700.038.3823540
TCTCGTA1700.038.3823541
ACCGTAG1650.038.1818230
CGTAGCA1750.037.28571332
CTCGTAT1800.036.25000442
AGACCGT1750.036.028
GAGCGGT1850.035.270276
AGAGCGG1850.035.270275
GTATGCC1800.035.00000445
CGGTTCA2050.032.926839
AGCGGTT2000.032.6257
AATGCCG1950.032.30769321
ATGCCGA1950.032.30769322
GAGACCG1900.031.97368427
CCGAGAC1900.031.97368425
CGGAAGA2100.031.0714281
Produced by FastQC (version 0.11.5)