FastQCFastQC Report
Mon 6 Jun 2016
ERR1379359.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379359.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770440
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGACAGATCTCGTATGCC13240.17184985203260475Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGC207.0325856E-444.99902730
CGTGACA1750.037.2849132
AGACCGT1750.035.9992228
TCTCGTA1800.035.00151441
CTCGTAT1750.034.7157942
CCGTGAC1950.033.46081531
GAGACCG1900.033.15717727
CGTATGC1850.032.83712844
ACCGTGA1950.032.30699530
AGAGCGG2000.031.5013625
GCGGTTC2000.031.4993178
AGCGGTT2000.031.4993177
TCGTATG1950.031.15317343
AAGAGCG2100.031.0727714
GACCGTG2050.030.73104129
CGGTTCA2100.029.999359
CGGAAGA2200.029.6642251
GAGCGGT2200.029.658456
CCGAGAC2200.029.6584525
GTATGCC2150.028.25520345
Produced by FastQC (version 0.11.5)