FastQCFastQC Report
Mon 6 Jun 2016
ERR1379357.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379357.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808970
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCTGTAATCTCGTATGCC15860.1960517695340989Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA2100.038.57259441
CGTATGC2100.038.5702144
GACCGTC2200.036.81701729
CTCGTAT2250.036.0010942
AGACCGT2350.034.46699528
ACCGTCT2350.034.46699530
GCGGTTC2400.033.7489368
AGAGCGG2500.033.3051225
CGTCTGT2450.033.0601832
TCGTATG2450.033.0601843
GAGCGGT2600.032.02026
CGGTTCA2550.031.7637049
AGCGGTT2650.031.4141037
GAGACCG2600.031.1528627
CCGAGAC2600.031.1528625
ATCTCGT2650.030.56696340
CCGTCTG2750.029.45361531
GTATGCC2750.029.45361545
AATCTCG2900.028.70776639
CGGAAGA3150.027.1470321
Produced by FastQC (version 0.11.5)