FastQCFastQC Report
Mon 6 Jun 2016
ERR1379355.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379355.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035447
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATACTCATCTCGTATGCC12280.11859612322021311Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAC1300.039.8070131
GACCGAT1400.035.35653729
CGATACT1500.034.49940532
ACCGATA1450.034.13734430
TCGTATG1600.032.34475743
AGCGGTT1600.032.3431937
CGTATGC1700.030.44212244
GCGGTTC1750.029.5709238
CTCGTAT1800.028.75089542
TCTCGTA1800.028.74950641
CGGTTCA1900.027.2363729
GAGACCG1900.027.23637227
AATGCCG1850.026.75629621
AAGAGCG1950.026.540574
GAGCGGT1950.026.540576
AGAGCGG1950.026.540575
CGGAAGA2000.025.8770541
AGACCGA1950.025.3841828
ATGCCGA2050.025.24346722
ATCTCGT2200.023.52232440
Produced by FastQC (version 0.11.5)