FastQCFastQC Report
Mon 6 Jun 2016
ERR1379350.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379350.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844321
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTATCTATCTCGTATGCC12260.14520543726852703Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2200.038.86261444
TCTCGTA2200.038.86261441
CCGGTAT2150.038.7199131
CTCGTAT2250.037.99942
CGGTATC2250.037.99932
AGCGGTT2350.037.339447
ACCGGTA2300.036.19470230
GACCGGT2300.036.19470229
GAGACCG2300.036.19470227
TATCTCG2400.035.62405839
GTATGCC2600.032.8837545
AGACCGG2550.032.646228
GAGCGGT2700.032.5029956
ATCTCGT2650.032.263340
TCGTATG2650.032.263343
AAGAGCG2800.032.147724
GCGGTTC2750.031.908258
AGAGCGG2800.031.3440285
CGGTTCA2800.031.3384639
CCGAGAC2800.030.53491225
Produced by FastQC (version 0.11.5)