FastQCFastQC Report
Mon 6 Jun 2016
ERR1379348.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379348.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788652
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCATCAATCTCGTATGCC11510.14594523313197708Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1350.036.6663641
CTCGTAT1400.035.35684242
CGTATGC1600.032.34347544
CCGAGAC1600.032.34347525
TCGTATG1550.031.93521543
ACCGTCA1700.031.76443730
ATCTCGT1650.031.36337340
CCGTCAT1750.029.57117831
AATGCCG1750.029.57117821
GAGACCG1750.029.57117827
ATGCCGA1750.029.57117822
GACCGTC1800.028.74975829
CGGTTCA1800.028.7497589
AGACCGT1800.028.74975828
CGTCATC1800.028.74975832
AAGAGCG1850.027.9745124
GCGGTTC1850.027.9727388
AGCGGTT1850.027.9727387
GAGCGGT1950.026.5399196
GTATGCC1950.026.53823745
Produced by FastQC (version 0.11.5)