FastQCFastQC Report
Mon 6 Jun 2016
ERR1379346.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379346.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1165877
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACTACTATCTCGTATGCC13270.11381989695310912Illumina Paired End PCR Primer 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1950.040.39169742
TCTCGTA1950.040.38996541
TATCTCG2050.038.41807639
CGTATGC2100.037.50175544
TCGTATG2100.037.50175543
GACCGAC2100.037.49853529
ACCGACT2100.037.49853530
CCGACTA2100.037.49853531
CGACTAC2200.035.7940632
GAGCGGT2300.035.2175256
GCGGTTC2300.034.237798
AGCGGTT2350.033.5093277
ATCTCGT2550.030.8851240
CGGTTCA2650.030.5648449
AGACCGA2700.029.99882728
AATGCCG2650.029.7158221
GTATGCC2750.029.4559245
AGAGCGG2750.029.455925
AAGAGCG2700.029.168034
CGGAAGA2850.027.632871
Produced by FastQC (version 0.11.5)