FastQCFastQC Report
Mon 6 Jun 2016
ERR1379345.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379345.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754019
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCATACTATCTCGTATGCC9060.1201561233868112Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAC1450.041.89494732
CTCGTAT1450.040.3432842
TATCTCG1500.038.99850539
TCTCGTA1500.038.99850541
AGACCGC1600.037.96729728
TCGTATG1550.037.74048643
GACCGCA1650.036.81677229
CGTATGC1600.036.561144
AGCGGTT1750.035.9986237
GAGACCG1700.035.73392527
CCGCATA1700.035.73392531
ACCGCAT1800.034.9986630
ATCTCGT1700.034.41044640
GTATGCC1700.034.41044645
GAGCGGT1850.034.0595256
GCGGTTC1850.034.052758
CGGTTCA2000.032.623759
AGAGCGG1950.032.3150255
AAGAGCG2050.030.7386844
CCGAGAC2050.030.7305325
Produced by FastQC (version 0.11.5)