FastQCFastQC Report
Mon 6 Jun 2016
ERR1379343.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379343.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1092914
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACTACATCTCGTATGCC16640.15225351674514187Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA2650.042.4518832
ACCGTAC2650.042.4518830
GACCGTA2700.041.66573329
CCGTACT2700.041.66573331
AGACCGT2700.041.66573328
CGTATGC2850.038.68511644
TCGTATG2850.038.68511643
CTCGTAT2850.038.68334642
GAGACCG2950.038.1347427
TCTCGTA2900.038.0181341
GTACTAC3050.036.8844233
GAGCGGT3250.034.6177756
CCGAGAC3300.034.09014525
CATCTCG3250.033.92386639
AGCGGTT3400.033.0874987
CGGAAGA3550.032.9600261
ATCTCGT3450.032.6094640
GCGGTTC3550.031.6894328
CGGTTCA3550.031.6894329
CGAGACC3650.031.43765426
Produced by FastQC (version 0.11.5)