FastQCFastQC Report
Mon 6 Jun 2016
ERR1379341.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379341.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779936
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTCACATATCTCGTATGCC16200.20770935051081116Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2250.037.9996244
GACCGTC2250.037.9996229
TCTCGTA2250.037.9996241
TATCTCG2300.037.17354239
TCGTATG2350.036.38261843
CGTCACA2400.035.62464532
ATCTCGT2500.034.1996640
CTCGTAT2500.034.1996642
AGACCGT2500.034.1996628
ACCGTCA2500.034.1996630
GTATGCC2650.033.9619345
CGGTTCA2600.032.884299
AGCGGTT2750.032.7269487
GCGGTTC2800.032.142548
GAGACCG2750.031.09060127
TACGGGG300.00514650729.999743
GAGCGGT3100.029.7596766
AAGAGCG3100.029.033834
AGAGCGG3150.028.5729735
CCGTCAC3000.028.49971631
Produced by FastQC (version 0.11.5)