FastQCFastQC Report
Mon 6 Jun 2016
ERR1379340.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379340.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences983816
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATAGTAATCTCGTATGCC13140.13356156029176186Illumina Paired End PCR Primer 2 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAG1950.041.53761731
CGATAGT1950.041.53761732
ACCGATA2150.037.67365330
CTCGTAT2200.036.81743642
TCTCGTA2200.036.81743641
TCGTATG2200.036.81743643
GACCGAT2250.035.99926829
CGTATGC2350.035.42481244
GAGCGGT2400.034.690326
CGGTTCA2400.034.68689
AGCGGTT2400.034.68687
GCGGTTC2450.033.97898
ATCTCGT2500.032.3993440
AGAGCGG2650.031.4192455
GAGACCG2600.031.15321227
ATGCCGA2700.030.83270622
AGACCGA2700.029.99939228
AATGCCG2950.028.98246221
GTATGCC2800.028.92798245
CGGAAGA2900.028.712151
Produced by FastQC (version 0.11.5)