FastQCFastQC Report
Mon 6 Jun 2016
ERR1379339.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379339.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116030
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAGATAATCTCGTATGCC13860.12419020994059299Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGT1100.036.81792428
CGTAGAT1050.036.42831832
GACCGTA1150.035.21714829
CCGTAGA1350.031.66644531
ACCGTAG1300.031.1536330
TCTCGTA1400.027.3212441
CGGAAGA1500.027.0022321
CTCGTAT1450.026.37912642
AGAGCGG1550.026.1300225
CGGTTCA1550.026.128859
GCGGTTC1650.025.908918
CGTATGC1500.025.4998244
TCGTATG1500.025.4998243
GAGCGGT1700.023.824436
ATCTCGT1650.023.18165840
AAGAGCG1750.023.1437324
AGCGGTT1850.021.8917397
CCGAGAC1750.021.8569925
AATCTCG2400.019.68736339
GAGACCG1950.019.61524827
Produced by FastQC (version 0.11.5)