FastQCFastQC Report
Mon 6 Jun 2016
ERR1379336.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379336.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886344
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAG1900.035.52581431
CGATAGA1900.035.52581432
ACCGATA1950.034.61489530
GACCGAT2050.032.92636529
CTCGTAT2150.032.4422642
CGTATGC2200.030.68219844
AGAGCGG2350.030.6403035
AGCGGTT2450.029.387347
TCGTATG2400.029.06285943
TCTCGTA2350.028.72299841
GAGCGGT2550.028.2371396
GCGGTTC2550.028.2348948
TATCTCG2450.027.5506339
ATCTCGT2600.026.82654240
CGGTTCA2800.026.5174839
AGACCGA2650.026.32038128
AATGCCG2850.025.26280221
CGGAAGA3050.024.3471451
GAGACCG2900.024.05138627
ATGCCGA3050.023.60622422
Produced by FastQC (version 0.11.5)