FastQCFastQC Report
Mon 6 Jun 2016
ERR1379333.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379333.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237670
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA302.155919E-645.00694341
CGTATGC302.1590458E-644.9974844
GACCGTT302.1590458E-644.9974829
TCGTATG302.1590458E-644.9974843
CCGTTTA302.1590458E-644.9974831
CTCGTAT356.2208E-638.5773842
GCGGTTC356.229797E-638.5692638
AGACCGT356.229797E-638.56926328
CGTTTAG458.6651744E-734.9980432
GAGACCG401.55598E-533.74810827
CCGAGAC401.55598E-533.74810825
ACCGTTT401.55598E-533.74810830
CGCAGTT300.0051299830.0109461
CATCTCG453.475658E-530.00463139
AGCGGTT453.4806566E-529.998327
GTATGCC453.4806566E-529.9983245
AGAGCGG453.4806566E-529.998325
ATCTCGT507.1274364E-527.00416840
AAGAGCG507.137658E-526.9984864
CGGTTCA507.137658E-526.9984869
Produced by FastQC (version 0.11.5)