FastQCFastQC Report
Mon 6 Jun 2016
ERR1379330.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379330.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363541
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTA451.9228537E-839.99816532
GACCGGT451.9228537E-839.99816529
TCTCGTA451.9228537E-839.99816541
TCGTATG451.9228537E-839.99816543
ATCTCGT504.865251E-835.99835240
CTCGTAT504.865251E-835.99835242
CGGTTCA504.865251E-835.9983529
TGCCGAG504.865251E-835.99835223
ACCGGTT551.1245538E-732.7257730
CGTATGC551.1245538E-732.7257744
GCGGTTC551.1245538E-732.725778
AGCGGTT551.1245538E-732.725777
AGACCGG551.1245538E-732.7257728
CCGGTTT551.1245538E-732.7257731
CGGAAGA602.4064502E-730.006881
AAGAGCG602.4093424E-730.002754
GAGCGGT602.4093424E-730.002756
AGAGCGG602.4093424E-730.002755
GAGACCG602.4122528E-729.99862527
CGCCCGG300.00514383729.99862513
Produced by FastQC (version 0.11.5)