FastQCFastQC Report
Mon 6 Jun 2016
ERR1379321.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379321.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324893
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT351.208391E-744.9996940
TCTCGTA351.208391E-744.9996941
CGTACAA403.4482036E-739.37473332
CTCGTAT458.6748696E-734.9997642
GACCGTA458.6748696E-734.9997629
CCGTACA458.6748696E-734.9997631
ACCGTAC501.9759445E-631.49978430
ATGCCGA501.9759445E-631.49978422
CGTATGC554.15308E-628.63616844
GCGGTTC554.15308E-628.6361688
AATGCCG554.15308E-628.63616821
AATCTCG554.15308E-628.63616839
AGCGGTT507.143201E-526.9998157
CGGTTCA608.168365E-626.2498199
TCGTATG608.168365E-626.24981943
AGAGCGG608.168365E-626.2498195
GAGACCG651.5194641E-524.23060227
AGACCGT651.5194641E-524.23060228
CCGAGAC651.5194641E-524.23060225
AAGAGCG602.4623412E-422.4998464
Produced by FastQC (version 0.11.5)