FastQCFastQC Report
Mon 6 Jun 2016
ERR1379317.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379317.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495842
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA800.039.37632441
GACCGGT800.039.37235329
CGTATGC900.035.0011844
CTCGTAT900.035.0011842
TCGTATG900.035.0011843
ACCGGTG900.034.99764630
GTATGCC950.033.15900845
CGGTGGT950.033.15566332
ATCTCGT1000.031.50105940
AGACCGG1000.031.4978828
CCGGTGG1000.031.4978831
GAGCGGT1200.030.0010076
AGCGGTT1200.029.9979847
GAGACCG1050.029.99798427
ATCGGAA300.005146160729.99798419
GCGGTTC1250.028.7980658
CCGAGAC1100.028.63443825
CGGAAGA1200.028.1401351
AGAGCGG1200.028.1259445
CGGTTCA1200.028.123119
Produced by FastQC (version 0.11.5)