FastQCFastQC Report
Mon 6 Jun 2016
ERR1379316.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379316.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478386
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAT403.4530785E-739.37426829
CGTATGC401.5588685E-533.7493744
TCGTATG401.5588685E-533.7493743
TCTCGTA453.487071E-529.99944341
CGATGGT453.487071E-529.99944332
CTCGTAT507.150716E-526.99949842
ACCGATG507.150716E-526.99949830
AGACCGA551.3667444E-424.54528
GAGACCG602.464899E-422.49958227
GAGCGGT602.464899E-422.4995826
AGCGCTC500.002262936222.4995827
CCGAGAC602.464899E-422.49958225
GATCTCG754.59238E-521.00180439
CCGATGG654.2337793E-420.76884531
CGGTTCA654.2337793E-420.7688459
CGGAAGA706.9712824E-419.287371
AGCGGTT706.9761893E-419.2853557
GTATGCC706.9761893E-419.28535545
ATCTCGT750.001109074217.99966440
GCGGTTC750.001109074217.9996648
Produced by FastQC (version 0.11.5)