FastQCFastQC Report
Mon 6 Jun 2016
ERR1379308.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379308.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447047
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC302.162773E-644.99921844
GCGGTTC356.240469E-638.570768
GAGCGGT356.240469E-638.570766
AGCGGTT401.5586278E-533.7494137
CCGAGAC401.5586278E-533.74941325
TCGTATG401.5586278E-533.74941343
AGAGCGG401.5586278E-533.7494135
TGCGTCC300.005144370729.9994816
ATCTCGT453.486537E-529.99947740
ACCGAGT453.486537E-529.99947730
GACCGAG507.1496295E-526.99952929
AGACCGA507.1496295E-526.99952928
CGGTTCA608.178024E-626.2495449
AATGCCG551.3665378E-424.54502921
CCGAGTT551.3665378E-424.54502931
ATGCCGA551.3665378E-424.54502922
AAGAGCG602.4626625E-422.5021274
CTCGTAT602.464528E-422.49960942
CGAGTTG602.464528E-422.49960932
TCTCGTA602.464528E-422.49960941
Produced by FastQC (version 0.11.5)