FastQCFastQC Report
Mon 6 Jun 2016
ERR1379307.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379307.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476605
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTC504.870344E-835.9986578
AGACCGA504.870344E-835.99865728
CGTATGC458.6882574E-734.99869544
CCGATGT551.12468115E-732.72948531
CGGTTCA551.125718E-732.726059
GACCGAT551.125718E-732.7260529
AGCGGTT551.125718E-732.726057
GAGACCG551.125718E-732.7260527
TGCCGAG551.125718E-732.7260523
ACCGCGC352.8185334E-432.141669
TCTCGTA501.9789786E-631.49882541
ACCGCAA300.0051452129.99888218
CCGCGCC453.4874894E-529.99888210
ATGCCGA602.4147448E-729.99888222
CGATGTG702.8769136E-828.93052732
ATCTCGT554.159425E-628.63529640
CTCGTAT554.159425E-628.63529642
ACCGATG803.3851393E-928.12395130
CACCGCG406.1621494E-428.1239518
GAGCGGT654.8649053E-727.6912756
Produced by FastQC (version 0.11.5)