FastQCFastQC Report
Mon 6 Jun 2016
ERR1379304.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379304.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456965
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT302.163055E-644.9986846
AGAGCGG401.5588292E-533.7490165
ATCTCGT352.816409E-432.14543540
CTCGTAT352.816409E-432.14543542
AGCGGTT352.8182182E-432.141927
CGCAATG352.8182182E-432.1419232
AATTCGG300.005144785629.99912316
TCTCGTA406.1575224E-428.12725841
CGTATGC406.1614654E-428.1241844
CGGTTCA406.1614654E-428.124189
AATGCCG406.1614654E-428.1241821
TCGTATG406.1614654E-428.1241843
ATGCCGA406.1614654E-428.1241822
GCGGTTC450.001225632324.9992718
AAGAGCG450.001225632324.9992714
GACCGCA500.002262921822.49934429
GAGACCG500.002262921822.49934427
AGACCGC500.002262921822.49934428
CCGAGAC500.002262921822.49934425
CCGCAAT500.002262921822.49934431
Produced by FastQC (version 0.11.5)