FastQCFastQC Report
Mon 6 Jun 2016
ERR1379298.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379298.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296274
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAC302.1600845E-644.99983229
CGACAGG302.1600845E-644.99983232
CGTATGC356.23276E-638.57128544
AGCGGTT356.23276E-638.5712857
GAGACCG356.23276E-638.57128527
TCGTATG356.23276E-638.57128543
CCGACAG356.23276E-638.57128531
ATCTCGT401.5567148E-533.7498740
GCGGTTC401.5567148E-533.749878
ACCGACA401.5567148E-533.7498730
TATCTCG401.5567148E-533.7498739
TCTCGTA401.5567148E-533.7498741
ATGCCGA401.5567148E-533.7498722
GAGCGGT453.482284E-529.9998876
AGACCGA453.482284E-529.99988728
CCGAGAC453.482284E-529.99988725
AGAGCGG453.482284E-529.9998875
TGCCGAG453.482284E-529.99988723
AATGCCG554.151356E-628.63625721
AAGAGCG507.1409624E-526.9998994
Produced by FastQC (version 0.11.5)