FastQCFastQC Report
Mon 6 Jun 2016
ERR1379297.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379297.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489139
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT302.1625965E-645.0010226
GCGGTTC356.244336E-638.5683638
AGCGGTT356.244336E-638.5683637
ATCTCGT401.5584983E-533.75076740
CGTATGC401.5595897E-533.7473244
CGGTTCA401.5595897E-533.747329
GACCGCA401.5595897E-533.7473229
CGGAAGA453.474095E-530.0160271
ACCGCAA453.4886794E-529.99761430
AGACCGC453.4886794E-529.99761428
TCGTATG453.4886794E-529.99761443
GAGACCG507.1540046E-526.99785427
TCTCGTA507.1540046E-526.99785441
CGCAAGG507.1540046E-526.99785432
CCGCAAG507.1540046E-526.99785431
TGCCGAG507.1540046E-526.99785423
CTCGTAT551.3673712E-424.54350342
TATCTCG602.4626133E-422.50281339
AGAGCGG602.4643185E-422.5005115
ATGCCGA602.4660246E-422.4982122
Produced by FastQC (version 0.11.5)