FastQCFastQC Report
Mon 6 Jun 2016
ERR1379294.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379294.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359523
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTC650.041.537058
GAGCGGT650.041.537056
TCTCGTA603.6379788E-1241.248641
CGTGTAG603.6379788E-1241.248632
CGGTTCA700.038.5701189
AGCGGTT700.038.5701187
CTCGTAT659.094947E-1238.0756342
AGAGCGG751.8189894E-1235.9987755
GACCGTG702.1827873E-1135.3559429
CCGTGTA702.1827873E-1135.3559431
AATGCCG702.1827873E-1135.3559421
AGACCGT702.1827873E-1135.3559428
ATGCCGA702.1827873E-1135.3559422
ATCTCGT754.7293724E-1132.9988840
AGTGGCG352.8171093E-432.14176610
CGGAAGA855.456968E-1231.7901541
AAGAGCG855.456968E-1231.7680444
CGTATGC855.456968E-1231.76362644
CCGAGAC801.0004442E-1030.9364525
CGAGTAG300.00514346329.9989835
Produced by FastQC (version 0.11.5)