FastQCFastQC Report
Mon 6 Jun 2016
ERR1379276.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379276.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518749
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT501.0786607E-940.5019076
CGTATGC501.0804797E-940.49800544
AGCGGTT501.0804797E-940.4980057
CGTGGCA552.744855E-936.8163732
AGAGCGG606.4155756E-933.751595
GACCGTG606.4210326E-933.7483429
CCGTGGC606.4210326E-933.7483431
TCGTATG606.4210326E-933.7483443
ATGCCGA606.4210326E-933.7483422
TGCCGAG606.4210326E-933.7483423
CGGTTCA651.4011675E-831.1523119
GGGACCG300.00514349930.00141334
GCGGTTC702.8810973E-828.9271478
AATGCCG702.8810973E-828.92714721
AGACCGT702.8810973E-828.92714728
ACCGTGG702.8810973E-828.92714730
CGGAAGA654.8463335E-727.7042961
CCGAGAC755.6294084E-826.99866925
AAGAGCG801.04999344E-725.3161334
TCTCGTA801.0509757E-725.31369441
Produced by FastQC (version 0.11.5)