FastQCFastQC Report
Mon 6 Jun 2016
ERR1379268.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379268.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences572215
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA351.210501E-745.041
CCGCATA351.210501E-745.031
CTCGTAT403.45417E-739.37542
GAGCGGT403.45417E-739.3756
AGACCGC403.45417E-739.37528
AGCGCGT250.002106226536.025
GACCGCA458.689767E-735.00000429
AGCGGTT458.689767E-735.0000047
GCGGTTC501.9793206E-631.58
CGCATAA501.9793206E-631.532
AATGCCG501.9793206E-631.521
ATGCCGA501.9793206E-631.522
AGAGCGG501.9793206E-631.55
ATCTCGT602.4153815E-729.99999840
GCGCGTC300.005145124629.99999826
AAGAGCG608.182156E-626.2499984
ACCGCAT608.182156E-626.24999830
TCGTATG608.182156E-626.24999843
CGTCCTC450.001225801725.00000229
CCGAGAC651.5220139E-524.2307725
Produced by FastQC (version 0.11.5)