FastQCFastQC Report
Mon 6 Jun 2016
ERR1379263.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379263.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672738
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACCG650.041.53880327
CTCGTAT700.038.57174742
GACCGGG751.8189894E-1235.9976229
TCGTATG803.6379788E-1233.75027543
GCGGTTC855.456968E-1231.7626088
CGGTTCA855.456968E-1231.7626089
CGTATGC901.2732926E-1130.0047144
AGACCGG901.2732926E-1130.00024828
CGCAAAC300.005147421729.99801615
ATGCCGA1001.8189894E-1229.2502422
AAGAGCG1053.6379788E-1227.8615154
ACCGGGT1004.5474735E-1126.99821730
AATGCCG1004.5474735E-1126.99821721
GAGCGGT1105.456968E-1226.5891516
TCTCGTA957.403287E-1026.05284741
CCGAGAC1101.5097612E-1024.54565625
CGGAAGA1201.6370905E-1124.3788261
ATCTCGT1052.3628672E-923.57162340
TGCCGAG1152.6375346E-1023.47845523
AGCGGTT1152.6557245E-1023.476717
Produced by FastQC (version 0.11.5)