FastQCFastQC Report
Mon 6 Jun 2016
ERR1379256.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379256.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences626206
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGT800.042.1874246
GACCGCC700.041.7856429
AGCGGTT850.039.7058147
CGTATGC750.038.9999344
CTCGTAT800.036.56243542
AAGAGCG1000.035.9999354
CTCGCAC250.002106396235.99993519
TCGTATG850.034.41170543
GCGGTTC1050.034.2856568
AGAGCGG1100.032.727225
ACCGCCT900.032.49994330
CGGTTCA1050.032.1428039
AGACCGC950.030.7894228
AATGCCG1100.030.68176721
CCGAGAC1050.029.99994925
ATGCCGA1150.029.34777322
CGGAAGA1200.028.1271951
TATCTCG1053.6379788E-1227.85709439
TCTCGTA1053.6379788E-1227.85709441
TGCCGAG1053.6379788E-1227.85709423
Produced by FastQC (version 0.11.5)