FastQCFastQC Report
Mon 6 Jun 2016
ERR1379245.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379245.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739580
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT403.456189E-739.37470242
CGATCGG403.456189E-739.37470232
TCGTATG552.746674E-936.81790543
CCGATCG501.980472E-631.49976331
CGCGCCT300.005146275329.99977311
CGTATGC554.162541E-628.63614844
GACCGAT554.162541E-628.63614829
GCGGTTC608.186864E-626.2498028
GAGCGGT608.186864E-626.2498026
TCTCGTA608.186864E-626.24980241
AGAGCGG651.5220883E-524.2322255
ACCGATC651.5228858E-524.23058530
AGACCGA651.5228858E-524.23058528
GATCGGA702.7014661E-522.4998333
CGGTTCA754.60005E-520.9998429
AGCGGTT754.60005E-520.9998427
GAGACCG807.558781E-519.68735127
CCGAGAC807.558781E-519.68735125
CGCCTGG1051.4442485E-619.2855713
GGCGGCT600.00650863518.74985939
Produced by FastQC (version 0.11.5)