FastQCFastQC Report
Mon 6 Jun 2016
ERR1379243.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379243.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737326
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC552.743036E-936.8216844
CTCGTAT606.4192136E-933.75320442
CGGTTCA606.4283086E-933.7486279
CCGGTAG606.4283086E-933.74862731
TATCTCG651.4026227E-831.15257839
AGACCGG651.4026227E-831.15257828
ACCGGTA702.8843715E-828.92739530
GCGGTTC702.8843715E-828.9273958
AGCGGTT755.635593E-826.9989017
GAGACCG856.4756023E-926.46951327
TCGTATG801.05175786E-725.31490343
CGGTAGG801.0531403E-725.3114732
TCTCGTA851.8900027E-723.82579241
GACCGGT851.8924766E-723.82256129
AATGCCG851.8924766E-723.82256121
GAGCGGT851.8924766E-723.8225616
CGGAAGA952.1062078E-823.691281
CCGAGAC952.1136657E-823.68324725
AGAGCGG903.2784737E-722.5036645
TGCCGAG955.5283454E-721.31492223
Produced by FastQC (version 0.11.5)