FastQCFastQC Report
Mon 6 Jun 2016
ERR1379224.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1379224.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773808
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCC1051.4446196E-619.28539
GGGCGGA750.001109937917.9996138
GCGCTCC901.86499E-417.4996228
TCTGTCG1053.1108728E-517.1424886
AGCGCTC800.001710409616.8746367
TGTCGCC1104.635223E-516.3632838
TCGCCCA1004.1655294E-415.749660510
CTGTCGC1156.7791654E-515.6518377
TTCACCG1000.007542712613.5014545
ACTAGAC2359.002179E-913.40396819
CGGGGTT1607.8030775E-512.6584311
ACCACGC1651.0422582E-412.2724639
CGGGCGT1300.003016068612.1174731
CGCTCCA1300.003020452312.1151249
CGAGTAG1350.0040018411.6679235
CCACGCC1751.803368E-411.5711810
CTCGCTC1600.001105583311.2519391
CGCTCTG1802.3398857E-411.2512123
AAAGCGC1600.001106181211.2512115
CGCCCAG1600.001107377911.24975811
Produced by FastQC (version 0.11.5)